Iban Seiliez

Responsabilités

Member of the management board of the research Unit NuMeA (Since 2015)

Thèmes de recherche

Autophagy and fish metabolism

My research aims to advance our understanding of the nutritional control of autophagy and its role in the regulation of intermediary metabolism in teleost fish. Due to the wide diversity of their lifestyles, metabolic features and phylogenetic position, these species represent relevant model organisms for studying the metabolic role as well as the evolutionary history of such a key function crucial for adaptation and survival.

Encadrement

Training of undergraduate students (from France and from abroad)

Thesis supervision: I. Belghit (Dec 2011 – Jan 2015); S. Séité (2016-2019); L. Lescat (2016-2019); H Liu, (2016-2020)

Projets

International projects

2014 - 2015      Univ of Alabama at Birmingham - Nutrition and Obesity Research Center (UAB NORC, USA), Pilot/Feasibility Grant. Renewal. Role of amino acids in the epigenetic regulation of muscle growth in determinate and indeterminate growing paradigms. (PI: P Biga)

2013 - 2014      Faculty Development Grant, Univ of Alabama at Birmingham (USA). Characterization of epigenetic regulation of indeterminate myogenesis and muscle atrophy. (PI: P Biga)

2013 - 2014      Univ of Alabama at Birmingham - Nutrition and Obesity Research Center (UAB NORC, USA), Pilot/Feasibility Grant. Role of amino acids in the epigenetic regulation of muscle growth in determinate and indeterminate growing paradigms. (PI: P Biga)

2009 - 2010      PHC PICASSO. Effect of Amino acids and IGF1 on the regulation of the ubiquitin-proteasome pathway in trout muscle cell cultures. (PI: I Seiliez)

2011 - 2016      EU FP7 ARRAINA Advanced Research Initiatives for Nutrition & Aquaculture. (PI : S Kaushik)

National projects

2017 - 2020      ANR Fish-and-Chap. Looking at the chaperone-mediated autophagy in fish. (PI: I Seiliez)

2017 -2018       ACI PHASE INRA. Autophagie, Physiologie Animale et Système d’Elevage. (PI: I Seiliez)

2016 - 2017      ACI PHASE INRA. Rôle of LAMP-2A in medaka. (PI: I Seiliez)

2013 - 2014      Univ of Pau (BQR).  Autophagy in European eel (Anguilla anguilla): relationship with the migratory behavior. (PI : V Bolliet)

2013 - 2014      ACI PHASE INRA. Role of autophagy in glucose utilization in rainbow trout (PI: I Seiliez)

2011 - 1012      ACI PHASE INRA. Dietary methionine and muscle growth in trout  (PI: I Seiliez)

2009 - 2012      ANR Jeune Chercheur CHAAINS. Low utilisation of dietary carbohydrates in carnivorous rainbow trout: role of amino acids, glucose and insulin interactions? (PI: S skiba)

2009 - 2012      ANR Blanc MYOTROPHY. Role of myostatin in the intracellular pathways regulating the balance between atrophy and hypertrophy in skeletal muscle. (PI: B Piccard)

2006 - 2008      Région Aquitaine (France). Role of amino acids on the transcriptional activity of insulin in rainbow trout. (PI: I Seiliez)

2006 - 2007      ACI PHASE INRA. Control of muscle proteolysis in fish and chick. (PI: I Seiliez)

Projects funded by Industrial partners

2008 - 2010         Rhodimet Research Grant (ADISSEO). Role of sulphur amino acids in controlling metabolism, growth and quality. (PI: S Tesseraud)

2017 - 2019 Evonik Industries. Short and long term metabolic consequences of dietary methionine deficiency in rainbow trout (PI: I Seiliez)

Publications

59 publications in international Peer-reviewed journals (h-index: 25; Sum of the Times Cited: 5143) and 21 oral communications + 17 Posters in national and international conferences.

Séité S, Mourier A, Camougrand N, Salin B, Cláudia Figueiredo-Silva A, Fontagné-Dicharry S, Panserat S, Seiliez I. (2018). Dietary methionine deficiency affects oxidative status, mitochondrial integrity and mitophagy in the liver of rainbow trout (Oncorhynchus mykiss). Sci Rep. Accepted.

Lescat L, Herpin A, Mourot B, Véron V, Guiguen Y, Bobe J, Seiliez I. (2018). CMA restricted to mammals and birds: myth or reality? Autophagy. Accepted.

Arevalo E, Panserat S, Seiliez I, Larrañaga A, Bardonnet A. (2018). Effect of food shortage and temperature on age 0+ salmonids: a contribution to predict the effects of climate change. J Fish Biol. 92(3):642-652.

Bolliet V, Labonne J, Olazcuaga L, Panserat S, Seiliez I. (2017). Modeling of autophagy-related gene expression dynamics during long term fasting in European eel (Anguilla anguilla). Sci Rep. 7(1):17896.

Biga PR, Latimer MN, Froehlich JM, Gabillard JC, Seiliez I. (2017). Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes. Biol Open. 6(11):1720-1725.

Latimer M, Sabin N, Le Cam A, Seiliez I, Biga P, Gabillard JC. (2017). miR-210 expression is associated with methionine-induced differentiation of trout satellite cells. J Exp Biol. 220(Pt 16):2932-2938.

Seiliez, I., Belghit, I., Gao, Y., Skiba-Cassy, S., Dias, K., Cluzeaud, M., Rémond, D., Hafnaoui, N., Salin, B., Camougrand, N., Panserat, S. (2016). Looking at the metabolic consequences of the colchicine-based in vivo autophagic flux assay. Autophagy. 12(2):343-56.

Klionsky, D.J. et al. (2467 co-authors including Seiliez, I.) (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 12(1):1-222.

Skiba-Cassy, S., Geurden, I., Panserat, S., Seiliez, I. (2016). Dietary methionine imbalance alters the transcriptional regulation of genes involved in glucose, lipid and amino acid metabolismin the liver of rainbow trout (Oncorhynchus mykiss). Aquaculture. 454: 56-65.

Dai, W.W., Panserat, S., Kaushik, S., Terrier, F., Plagnes-Juan, E., Seiliez, I., Skiba-Cassy, S. (2016). Hepatic fatty acid biosynthesis is more responsive to protein than carbohydrate in rainbow trout during acute stimulations. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology. 310(1):R74-86.

Dai, W. W., Panserat, S., Plagnes- Juan, E., Seiliez, I., Skiba-Cassy, S. (2015). Amino acids attenuate insulin action on gluconeogenesis and promote fatty acid biosynthesis via mTORC1 signaling pathway in trout hepatocytes. Cellular physiology and biochemistry. 36, 1084-1100.

Marandel, L., Seiliez, I., Veron, V., Skiba-Cassy, S., Panserat, S. (2015). New insights into the nutritional regulation of gluconeogenesis in carnivorous rainbow trout (Oncorhynchus mykiss): a gene duplication trail. Physiol Genomics. 47(7):253-63.

Gabillard, J.C., Biga, P.R., Rescan, P.Y., Seiliez, I. 2013. Revisiting the paradigm of myostatin in vertebrates: insights from fishes. Gen Comp Endocrinol. 194:45-54.

Seiliez, I., Medale, F., Aguirre, P., Larquier, M., Lanneretonne, L., Alami-Durante, H., Panserat, S., Skiba-Cassy, S. 2013. Effect of dietary carbohydrate to protein ratio on the expression of hepatic and muscle metabolism- and growth-related factors in zebrafish (Danio rerio). Zebrafish. 10(2):237-48.

Skiba-Cassy, S., Panserat, S., Larquier, M., Dias, K., Surget, A., Plagnes-Juan, E.,  Kaushik, S., Seiliez, I.  2012. Apparent low ability of liver and muscle to adapt to variation of dietary carbohydrate-to-protein ratio in rainbow trout. Br J Nutr. 6:1-14.

Seiliez, I., Froehlich, J.M., Marandel, L., Gabillard, J.C., Biga, P.R. 2015. Evolutionary history and epigenetic regulation of the three paralogous pax7 genes in rainbow trout. Cell Tissue Res. 359(3):715-27.

Seiliez, I., Dias, K., Cleveland, B.M. 2014. Contribution of the autophagy-lysosomal and ubiquitin-proteasomal proteolytic systems to total proteolysis in rainbow trout (Oncorhynchus mykiss) myotubes. Am J Physiol Regul Integr Comp Physiol. 307(11):R1330-7.

Dai, W., Panserat, S., Terrier, F., Seiliez, I., Skiba-Cassy, S. 2014. Acute rapamycin treatment improved glucose tolerance through inhibition of hepatic gluconeogenesis in rainbow trout (Oncorhynchus mykiss). Am J Physiol Regul Integr Comp Physiol. 307(10):R1231-8.

Rodriguez, J., Vernus, B., Chelh, I., Cassar-Malek, I., Cottin, P., Gabillard, J.C., Hadj Sassi, A., Seiliez, I., Picard, B., Bonnieu, A. 2014. Myostatin and the skeletal muscle atrophy and hypertrophy signaling pathways. Cell Mol Life Sci. 71(22):4361-71.

Belghit,I., Skiba-Cassy, S., Geurden, I., Dias, K., Surget, A., Kaushik, S., Panserat, S., Seiliez, I. 2014. Dietary methionine availability affects the main factors involved in muscle protein turnover in rainbow trout (Oncorhynchus mykiss). Br J Nutr. 112(4):493-503.

Froehlich, J.M., Seiliez, I., Gabillard, J.C., Biga, P.R. 2014. Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages. J Vis Exp. (86), 1-12.

Mennigen, J.A., Martyniuk, C.J., Seiliez, I., Panserat, S., Skiba-Cassy, S. 2014. Metabolic consequences of microRNA-122 inhibition in rainbow trout, Oncorhynchus mykiss. BMC Genomics.15:70.

Mennigen, J.A., Plagnes-Juan, E., Figueredo-Silva, C.A., Seiliez, I., Panserat, S., Skiba-Cassy, S. 2014. Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 169:16-24.

Belghit, I., Panserat, S., Sadoul, B., Dias, K., Skiba-Cassy, S., Seiliez, I. 2013. Macronutrient composition of the diet affects the feeding-mediated down regulation of autophagy in muscle of rainbow trout (O. mykiss). PLoS One. 8(9):e74308.

Dai, W., Panserat, S., Mennigen, J.A., Terrier, F., Dias, K., Seiliez, I., Skiba-Cassy, S. 2013. Post-prandial regulation of hepatic glucokinase and lipogenesis requires the activation of TORC1 signalling in rainbow trout (Oncorhynchus mykiss). J Exp Biol. 216(Pt 23):4483-92.

Seiliez, I., Taty Taty, G.C., Bugeon, J., Dias, K., Sabin, N., Gabillard, JC. 2013. Myostatin induces atrophy of trout myotubes through inhibiting the TORC1 signaling and promoting Ubiquitin-Proteasome and Autophagy-Lysosome degradative pathways. Gen Comp Endocrinol. 186C:9-15.

Klionsky, D.J. and 1268 others (including Seiliez, I.). 2012. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8(4):445-544.

Mennigen, J.A., Panserat, S., Larquier, M., Plagnes-Juan, E., Medale, F., Seiliez, I., Skiba-Cassy, S. Postprandial regulation of hepatic microRNAs predicted to target the insulin pathway in rainbow trout. PLoS One. 2012;7(6):e38604.

Boussaid-Om Ezzine, S., Métayer-Coustard, S., Collin, A., Rideau, N., Leterrier, C., Bouvarel, I., Seiliez, I., Tesseraud, S. 2012. Regulators of protein metabolism are affected by cyclical nutritional treatments with diets varying in protein and energy content. J Nutr Biochem, 23(11):1467-73.

Seiliez, I., Gabillard, J.C., Riflade, M., Sadoul, B., Dias, K., Avérous, J., Tesseraud, S., Skiba, S., Panserat, S. 2012. Amino acids downregulate the expression of several autophagy-related genes in rainbow trout myoblasts. Autophagy, 8(3): 364 – 375.

Seiliez, I., Sabin, N., Gabillard, J.C. 2012. Myostatin inhibits proliferation but not differentiation of trout myoblasts. Mol Cell Endocrinol, 351(2):220-6.

Polakof, S., Skiba-Cassy, S., Kaushik, S., Seiliez, I., Soengas, J.L., Panserat, S. 2011. Glucose and lipid metabolism in the pancreas of rainbow trout is regulated at the molecular level by nutritional status and carbohydrate intake. J Comp Physiol B, 182(4):507-16.

Averous, J., Gabillard, J.C., Seiliez, I., Dardevet, D. 2011. Leucine limitation regulates myf5 and myoD expression and inhibits myoblast differentiation. Exp Cell Res, 318(3):217-27.

Seiliez, I., Sabin, N., Gabillard, J.C. 2011. FoxO1 is not a key transcription factor in the regulation of myostatin (mstn-1a and mstn-1b) gene expression in trout myotubes. Am J Physiol Regul Integr Comp Physiol, 301(1):R97-104.

Seiliez, I., Panserat, S., Skiba-Cassy, S., Polakof, S. 2011. Effect of acute and chronic insulin administrations on major factors involved in the control of muscle protein turnover in rainbow trout (Oncorhynchus mykiss). Gen Comp Endocrinol, 172(3):363-70.

Seiliez, I., Panserat, S., Lansard, M., Polakof, S., Plagnes-Juan, E., Surget, A., Dias, K., Larquier, M., Kaushik, S., Skiba-Cassy, S. 2011. Dietary carbohydrate to protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal. Am J Physiol Regul Integr Comp Physiol, 300(3):R733-43.

Lansard, M., Panserat, S., Plagnes-Juan, E., Dias, K., Seiliez, I., Skiba-Cassy, S. 2011. L-leucine, L-methionine, and L-lysine are involved in the regulation of intermediary metabolism-related gene expression in rainbow trout hepatocytes. J Nutr, 141(1):75-80.

Métayer-Coustard, S., Mameri, H., Seiliez, I., Crochet, S., Crépieux, P., Mercier, Y., Geraert, P.A., Tesseraud, S. 2010. Methionine deprivation regulates the S6K1 pathway and protein synthesis in avian QM7 myoblasts without activating the GCN2/eIF2 alpha cascade. J Nutr, 140(9):1539-45.

Seiliez, I., Gutierrez, J., Salmerón, C., Skiba-Cassy, S., Chauvin, C., Dias, K., Kaushik, S., Tesseraud, S., Panserat, S. 2010. An in vivo and in vitro assessment of autophagy-related gene expression in muscle of rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol B Biochem Mol Biol, 157(3):258-66.

Lansard, M., Panserat, S., Plagnes-Juan, E., Seiliez, I., Skiba-Cassy, S. 2010. Integration of insulin and amino acid signals that regulate hepatic metabolism-related gene expression in rainbow trout: role of TOR. Amino Acids, 39(3):801-10.

Lansard, M., Panserat, S., Seiliez, I., Polakof, S., Plagnes-Juan, E., Geurden, I., Médale, F., Kaushik, S., Corraze, G., Skiba-Cassy, S. 2009. Hepatic protein kinase B (Akt)-target of  rapamycin (TOR)-signalling pathways and intermediary metabolism in rainbow trout  (Oncorhynchus mykiss) are not significantly affected by feeding plant-based diets. Br J Nutr, 102(11):1564-73.

Panserat, S., Skiba-Cassy, S., Seiliez, I., Lansard, M., Plagnes-Juan, E., Vachot, C., Aguirre, P., Larroquet, L., Chavernac, G., Medale, F., Corraze, G., Kaushik, S., Moon, T.W. 2009. Metformin improves postprandial glucose homeostasis in rainbow trout fed dietary carbohydrates: a link with the induction of hepatic lipogenic capacities? Am J Physiol Regul Integr Comp Physiol, 297(3):R707-15.

Kaushik, S.J. & Seiliez, I. 2009. Protein and amino acid nutrition and metabolism in fish: current knowledge and future needs. Aquaculture Research, 41: 322-332.

Tesseraud, S., Métayer Coustard, S., Collin, A., Seiliez, I. 2009. Role of sulfur amino acids in controlling nutrient metabolism and cell functions: implications for nutrition. British Journal of Nutrition, 101, 1132–1139.

Tesseraud, S., Bouvarel, I., Collin, A., Audouin, E., Crochet, S., Seiliez, I., Leterrier, C. 2009. Daily variations in dietary lysine content alter the expression of genes related to proteolysis in chicken pectoralis major muscle. J Nutr, 139(1):38-43.

Chelh, I., Rodriguez, J., Bonnieu, A., Cassar-Malek, I., Cottin, P., Gabillard, J.C., Leibovitch, S., Sassi, A.H., Seiliez, I., Picard, B. 2009. Myostatin: a negative regulator of muscle mass in vertebrates. Productions Animales, 22(5):397-408.

Plagnes-Juan, E., Lansard, M., Seiliez, I., Médale, F., Corraze, G., Kaushik, S., Panserat, S., Skiba-Cassy, S. 2008. Insulin regulates the expression of several metabolism related genes in the liver and primary hepatocytes of rainbow trout (Oncorhynchus mykiss). J Exp Biol, 211(Pt 15):2510-8.

Seiliez, I., Gabillard, J.C., Skiba-Cassy, S., Garcia-Serrana, D., Gutierrez, J., Kaushik, S., Panserat, S., Tesseraud, S. 2008. An in vivo and in vitro assessment of TOR signalling cascade in rainbow trout (Oncorhynchus mykiss). Am J Physiol - Regul Integr Comp Physiol, 295: R329–R335.

Seiliez, I., Panserat, S., Skiba-Cassy, S., Fricot, A., Vachot, C., Kaushik, S., Tesseraud, S. 2008. Feeding status regulates the polyubiquitination step of the ubiquitin proteasome-dependent proteolysis in rainbow trout (Oncorhynchus mykiss) Muscle. J Nutr, 138(3): 487-91.

Métayer, S., Seiliez, I., Collin, A., Duchêne, S., Mercier, Y., Geraert, P.A., Tesseraud, S. 2008. Mechanisms through which sulfur amino acids control protein metabolism and oxidative status. J Nutr Biochem, 19(4): 207-15.

Tesseraud, S., Métayer-Coustard, S., Boussaid, S., Crochet, S., Audouin, E., Derouet, M., Seiliez, I. 2007. Insulin and amino acid availability regulate atrogin-1 in avian QT6 cells. Biochem Biophys Res Commun, 357(1):181-6.

Seiliez, I., Bruant, J.S., Zambonino Infante, J.L., Kaushik, S., Bergot, P. 2006. Effect of dietary phospholipid level on the development of gilthead sea bream (Sparus aurata) larvae fed a compound diet. Aquaculture Nutrition, 12; 372–378.

Seiliez, I., Thisse, B., Thisse, C. 2006. FoxA3 and Goosecoid Promote Anterior Neural Fate through Inhibition of Wnt8a Activity Before the Onset of Gastrulation. Dev Biol, 290(1):152-63.

Thisse, B., Heyer, V., Lux, A., Alunni, V., Degrave, A., Seiliez, I., Kirchner, J., Parkhill, J.P., Thisse, C. 2004. Spatial and temporal expression of the Zebrafish genome by large-scale in-situ Hybridization Screening. Methods Cell Biol, 77: 505-19.

Zheng, X., Seiliez, I., Hastings, N., Tocher, D.R., Panserat, S., Dickson, C.A., Bergot, P., Teale, A.J. 2004. Characterisation and comparison of fatty acyl Delta6-desaturase cDNAs from freshwater and marine teleost fish species. Comp Biochem Physiol, 139(2): 269-79.

Seiliez, I., Panserat, S., Corraze, G., Kaushik, S., Bergot, P. 2003. Cloning and nutritional regulation of a Delta6-desaturase-like enzyme in the marine teleost gilthead seabream (Sparus aurata). Comp Biochem Physiol,135(3):449-60.

Seiliez, I., Panserat, S., Kaushik, S., Bergot, P. 2001. Cloning, tissue distribution and nutritional regulation of a Delta6-desaturase-like enzyme in rainbow trout. Comp Biochem Physiol, 130(1): 83-93.

Paul, C., Seiliez, I., Thissen, J.P., Le Cam, A. 2000. Regulation of expression of the rat SOCS-3 gene in hepatocytes by growth hormone, interleukin-6 and glucocorticoids mRNA analysis and promoter characterization. Eur J Biochem, 267(19): 5849-57.

Kim, F.J., Seiliez, I., Denesvre, C., Lavillette, D., Cosset, F.L., Sitbon, M. 2000. Definition of an amino-terminal domain of the human T-cell leukemia virus type 1 envelope surface unit that extends the fusogenic range of an ecotropic murine leukemia virus. J Biol Chem, 275(31): 23417-20.